Coding

Part:BBa_K1096000:Design

Designed by: Ayten Yazgan Karatas   Group: iGEM13_ITU_MOBGAM_Turkey   (2013-08-29)


Gastric Intrinsic Factor (GIF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 523
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence is optimized for expressing in E. coli. And OmpA secretion signal sequence was added N terminus of the protein.

Source

Sequence of gene is obtained from homo sapiens genome.

References

Mathews, F. S. et al. (2007). Crystal structure of human intrinsic factor: cobalamin complex at 2.6 Å resolution. Proc. Natl. Acad. Sci. USA 104 (44), 17311-17316.